The processing of tissues in the histology laboratory involves four steps: fixation, dehydration, clearing, and infiltration. Each of these steps requires the use of chemicals that may be hazardous, including solvents. Deciding which chemical to use is governed by a variety of considerations, including cost, type of tissue to be processed, processing method to be employed, and safety factors. The chemicals used in the histology laboratory pose a variety of safety risks; consequently, it is important to employ safe handling practices.
Section 1: Introduction
About This Course
Section 2: Dehydration Agents
Preparing Tissues for Examination
Section 3: Clearing Agents
Section 4: Hazards of Common Histology Solvents
Section 5: Safety and Exposure Control
Gold Standard of Fixation Agents: Formaldehyde
Section 6: Hazard Communication Standard (HCS) and Globally Harmonized System for Labeling Chemicals
Components of the HCS
Section 7: Clinical Vignette
Preparing Tissue for Examination
Section 8: Conclusion
Leonard I. Levin, MS, MBA, RM(NRCM), M(ASCP), has a BS in biochemistry, MS in microbiology and immunology, and MBA in finance. Disclosure: Leonard I. Levin, MS, MBA, RM(NRCM), M(ASCP) has declared that no conflict of interest, Relevant Financial Relationship or Relevant Non-Financial Relationship exists.Instructor: Catherine J. Swift, MT(ASCP)
Catherine Swift, MT(ASCP) is the clinical laboratory science editor for Relias Learning. She has a Bachelor of Arts in English from Hastings College and a Bachelor of Science in Medical Technology from North Colorado Medical Center. She has experience working in hospital laboratories, clinic laboratories, and research experience in microbiology as well as clinical research trials involving patients and various drug therapies for osteoporosis, endometriosis and bone density at Creighton University School of Medicine. Disclosure: Catherine J. Swift, MT(ASCP) has declared that no conflict of interest, Relevant Financial Relationship or Relevant Non-Financial Relationship exists.
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